Fig. 8

Circ_005077 promoted ferroptosis in H9c2 cells via CyPA/p47phox. (A, E) Representative immunofluorescent staining and quantitative analysis of ferrous iron (orange, 400× magnification). (B, F) Representative immunofluorescent staining and quantitative analysis of LPO (green, 400× magnification). (C, G, H) Representative immunofluorescent staining and quantitative analysis of cellular ROS (green,400× magnification) and mitoROS (red, 400× magnification). (D, I) IF staining (600× magnification) was used to determine the surface area of NRVCs. (J, K) The transfection efficiency of p47phox overexpression p47phox and inhibition p47phox was demonstrated by Western blotting, and the corresponding quantification was exhibited. (L) LDH expression levels. Data were shown as mean ± SD of at least three independent experiments. *P < 0.05; **P < 0.01; *** P < 0.001