Fig. 7

Circ_005077 promoted ferroptosis in H9c2 cells via CyPA. H9c2 cells (C∼I) ) and primary neonatal rat ventricular cardiomyocytes (NRVCs) (G, H) were transfected with vector; OE-CyPA; OE-circ_005077; OE-circ_005077 + NC(co-transfected with OE-circ_005077 and NC); or OE-circ_005077 + Si-CyPA(co-transfected with OE-circ_005077 and Si-CyPA). (A) Transfection efficiency of CyPA overexpression and inhibition of CyPA demonstrated by Western blotting. The corresponding quantification was shown. (B) The relative expression of circ_005077 in H9c2 cells with inhibition or overexpression of CyPA is shown. (C) Representative TEM images of mitochondria in H9C2 cells (1,500×, upper row and magnified views at 6,000, lower row). (D) The changes in LDH levels. (E) The changes in GSH levels. (F) The changes in MDA levels. (G) The mRNA expression of ANP in NRVCs was evaluated using qRT-PCR. (H) qRT-PCR evaluated the mRNA expression of BNP in NRVCs. (I) Western blotting detecting TFRC, ACSL4, COX2, FTH1, and GPX4 protein levels. Data were shown as mean ± SD of at least three independent experiments. *P < 0.05; **P < 0.01; *** P < 0.001