Fig. 10

Circ_005077 regulated cardiac hypertrophy and fibrosis of myocardial tissue in vivo. AAV9-OE-circ_005077, AAV9-OE-NC, AAV9-SH-circ_005077, and AAV9-SH-NC were injected individually through the tail vein. (A) Relative expression of rno_circ_005077 in the heart, liver, kidney, lung, and spleen after overexpression or silencing of rno_circ_005077 with AAV9 injection. (B) Each rat’s heart weight and tibia length and the ratio of heart weight to tibia length (HW/TL) (C, D) Representative echocardiographic images from the respective groups of rats. Systolic and diastolic function in rat (LVID; d, LVPW; d, CO). (E, F) HE staining to assess the presence of hypertrophy in cardiomyocytes and statistical results of cross-sectional area (400× magnification); Masson staining to identify myocardial fibrosis and statistical results of the degree of fibrosis (200× magnification); Sirus red staining to determine collagen fiber deposition and statistical results of collagen content (200× magnification). (G) qPCR was used to identify hypertrophic marker and fibrosis genes in the indicated groups. AAV9: adeno-associated virus-9; n = 6 in each group. The data were displayed as the normalized mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001